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Increased immune cells in BALF from EtOH+ Synthetic cannabinoid receptor agonist -exposed mice measured by flow cytometry. BALF cells were isolated and stained with fluorophore-conjugated antibodies to identify key pulmonary immune cell subsets, including dendritic cells, exudative macrophages, alveolar macrophages, and neutrophils. The following antibodies were used: CD170 (Siglec-F)-FITC, Ly6G-PE, F4/80-AF700, CD11b-APC, CD11c-PC5.5, and a fixable viability dye (eFluor 450) (all from Thermo Fisher Scientific/eBioscience). Samples were acquired on a three-laser <t>CytoFLEX</t> flow cytometer (Beckman Coulter) and analyzed using FCS Express (version 7; DeNovo Software). At least 30,000 events were collected per sample. Gating strategies were applied to identify specific innate immune cell populations involved in pulmonary inflammation across treatment groups. Flow analyses per group, with a representative image for each group, are shown. *=0.05, **=0.001 and ***=0.0001. ns, not significant.
Three Color Cytoflex Flow Cytometer, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Increased immune cells in BALF from EtOH+ Synthetic cannabinoid receptor agonist -exposed mice measured by flow cytometry. BALF cells were isolated and stained with fluorophore-conjugated antibodies to identify key pulmonary immune cell subsets, including dendritic cells, exudative macrophages, alveolar macrophages, and neutrophils. The following antibodies were used: CD170 (Siglec-F)-FITC, Ly6G-PE, F4/80-AF700, CD11b-APC, CD11c-PC5.5, and a fixable viability dye (eFluor 450) (all from Thermo Fisher Scientific/eBioscience). Samples were acquired on a three-laser <t>CytoFLEX</t> flow cytometer (Beckman Coulter) and analyzed using FCS Express (version 7; DeNovo Software). At least 30,000 events were collected per sample. Gating strategies were applied to identify specific innate immune cell populations involved in pulmonary inflammation across treatment groups. Flow analyses per group, with a representative image for each group, are shown. *=0.05, **=0.001 and ***=0.0001. ns, not significant.
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Increased immune cells in BALF from EtOH+ Synthetic cannabinoid receptor agonist -exposed mice measured by flow cytometry. BALF cells were isolated and stained with fluorophore-conjugated antibodies to identify key pulmonary immune cell subsets, including dendritic cells, exudative macrophages, alveolar macrophages, and neutrophils. The following antibodies were used: CD170 (Siglec-F)-FITC, Ly6G-PE, F4/80-AF700, CD11b-APC, CD11c-PC5.5, and a fixable viability dye (eFluor 450) (all from Thermo Fisher Scientific/eBioscience). Samples were acquired on a three-laser <t>CytoFLEX</t> flow cytometer (Beckman Coulter) and analyzed using FCS Express (version 7; DeNovo Software). At least 30,000 events were collected per sample. Gating strategies were applied to identify specific innate immune cell populations involved in pulmonary inflammation across treatment groups. Flow analyses per group, with a representative image for each group, are shown. *=0.05, **=0.001 and ***=0.0001. ns, not significant.
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Increased immune cells in BALF from EtOH+ Synthetic cannabinoid receptor agonist -exposed mice measured by flow cytometry. BALF cells were isolated and stained with fluorophore-conjugated antibodies to identify key pulmonary immune cell subsets, including dendritic cells, exudative macrophages, alveolar macrophages, and neutrophils. The following antibodies were used: CD170 (Siglec-F)-FITC, Ly6G-PE, F4/80-AF700, CD11b-APC, CD11c-PC5.5, and a fixable viability dye (eFluor 450) (all from Thermo Fisher Scientific/eBioscience). Samples were acquired on a three-laser <t>CytoFLEX</t> flow cytometer (Beckman Coulter) and analyzed using FCS Express (version 7; DeNovo Software). At least 30,000 events were collected per sample. Gating strategies were applied to identify specific innate immune cell populations involved in pulmonary inflammation across treatment groups. Flow analyses per group, with a representative image for each group, are shown. *=0.05, **=0.001 and ***=0.0001. ns, not significant.
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Increased immune cells in BALF from EtOH+ Synthetic cannabinoid receptor agonist -exposed mice measured by flow cytometry. BALF cells were isolated and stained with fluorophore-conjugated antibodies to identify key pulmonary immune cell subsets, including dendritic cells, exudative macrophages, alveolar macrophages, and neutrophils. The following antibodies were used: CD170 (Siglec-F)-FITC, Ly6G-PE, F4/80-AF700, CD11b-APC, CD11c-PC5.5, and a fixable viability dye (eFluor 450) (all from Thermo Fisher Scientific/eBioscience). Samples were acquired on a three-laser <t>CytoFLEX</t> flow cytometer (Beckman Coulter) and analyzed using FCS Express (version 7; DeNovo Software). At least 30,000 events were collected per sample. Gating strategies were applied to identify specific innate immune cell populations involved in pulmonary inflammation across treatment groups. Flow analyses per group, with a representative image for each group, are shown. *=0.05, **=0.001 and ***=0.0001. ns, not significant.
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Increased immune cells in BALF from EtOH+ Synthetic cannabinoid receptor agonist -exposed mice measured by flow cytometry. BALF cells were isolated and stained with fluorophore-conjugated antibodies to identify key pulmonary immune cell subsets, including dendritic cells, exudative macrophages, alveolar macrophages, and neutrophils. The following antibodies were used: CD170 (Siglec-F)-FITC, Ly6G-PE, F4/80-AF700, CD11b-APC, CD11c-PC5.5, and a fixable viability dye (eFluor 450) (all from Thermo Fisher Scientific/eBioscience). Samples were acquired on a three-laser CytoFLEX flow cytometer (Beckman Coulter) and analyzed using FCS Express (version 7; DeNovo Software). At least 30,000 events were collected per sample. Gating strategies were applied to identify specific innate immune cell populations involved in pulmonary inflammation across treatment groups. Flow analyses per group, with a representative image for each group, are shown. *=0.05, **=0.001 and ***=0.0001. ns, not significant.

Journal: Frontiers in Immunology

Article Title: Cannabinoids and alcohol co-exposure modulate pathogen-induced pulmonary immune responses

doi: 10.3389/fimmu.2025.1539813

Figure Lengend Snippet: Increased immune cells in BALF from EtOH+ Synthetic cannabinoid receptor agonist -exposed mice measured by flow cytometry. BALF cells were isolated and stained with fluorophore-conjugated antibodies to identify key pulmonary immune cell subsets, including dendritic cells, exudative macrophages, alveolar macrophages, and neutrophils. The following antibodies were used: CD170 (Siglec-F)-FITC, Ly6G-PE, F4/80-AF700, CD11b-APC, CD11c-PC5.5, and a fixable viability dye (eFluor 450) (all from Thermo Fisher Scientific/eBioscience). Samples were acquired on a three-laser CytoFLEX flow cytometer (Beckman Coulter) and analyzed using FCS Express (version 7; DeNovo Software). At least 30,000 events were collected per sample. Gating strategies were applied to identify specific innate immune cell populations involved in pulmonary inflammation across treatment groups. Flow analyses per group, with a representative image for each group, are shown. *=0.05, **=0.001 and ***=0.0001. ns, not significant.

Article Snippet: Data acquisition was performed using a three-color CytoFLEX flow cytometer (Beckman Coulter, FL, USA).

Techniques: Flow Cytometry, Isolation, Staining, Software